∵A(x1,y1),B(x2,y2)双曲线y=? 2 x 上的点,∴x1y1=-2,x2y2=-2,∵直线y=kx(k<0)与双曲线y=? 2 x 交于A(x1,y1),B(x2,y2)两点,∴x1=-x2,y1=-y2,∴x1y2=-x1y1,x2y1=-x2y2,∴3x1y2-8x2y1=-3x1y1+8x2y2=(-3)×(-2)+8×(-2)=-10.故选B.